Journal: Thoracic Cancer

Article Title: Circ_0020123 plays an oncogenic role in non‐small cell lung cancer depending on the regulation of miR ‐512‐3p/ CORO1C

doi: 10.1111/1759-7714.14408

Figure Lengend Snippet: Circ_0020123 serves as a sponge for miR‐512‐3p in NSCLC cells. (a and b) The subcellular localization of circ_0020123 was analyzed. (c) StarBase database predicted the binding sites between circ_0020123 and miR‐512‐3p. (d) RT‐qPCR was applied to verify the overexpression efficiency of miR‐512‐3p in NSCLC cells. (e and f) Dual‐luciferase reporter assay was applied to confirm the target relation between circ_0020123 and miR‐512‐3p. (g and h) RIP assay was implemented to verify the spatial interaction between circ_0020123 and miR‐512‐3p in RISC. (i) RT‐qPCR was conducted to detect the level of miR‐512‐3p in NSCLC tumor tissues ( n = 39) and adjacent normal tissues ( n = 39). (j) The expression of miR‐512‐3p in NSCLC cell lines (A549, H1299 and PC‐9) and 16HBE was determined by RT‐qPCR. (k) The interference efficiency of anti‐miR‐512‐3p in NSCLC cells was tested via RT‐qPCR. (L and M) A549 and PC‐9 cells were transfected with si‐circ_0020123 alone or together with anti‐miR‐512‐3p, and the level of miR‐512‐3p was determined by RT‐qPCR. * p < 0.05

Article Snippet: We collected NSCLC tissue samples and adjacent normal lung tissue samples from 39 NSCLC patients at The Affiliated Nanhua Hospital.

Techniques: Binding Assay, Quantitative RT-PCR, Over Expression, Luciferase, Reporter Assay, Expressing, Transfection

Journal: Thoracic Cancer

Article Title: Circ_0020123 plays an oncogenic role in non‐small cell lung cancer depending on the regulation of miR ‐512‐3p/ CORO1C

doi: 10.1111/1759-7714.14408

Figure Lengend Snippet: CORO1C is a target of miR‐512‐3p in NSCLC cells. (a) The binding sequence between miR‐512‐3p and CORO1C was predicted by TargetScan database. (b and c) The binding relation between miR‐512‐3p and CORO1C was tested via dual‐luciferase reporter assay. (d and e) RIP assay was employed to confirm the target relation between miR‐512‐3p and CORO1C. (f and g) RT‐qPCR and western blot assay were utilized to measure the expression of CORO1C in NSCLC tissues and adjacent normal tissues in mRNA and protein levels. (h) Western blot assay was utilized to measure the protein level of CORO1C in NSCLC cell lines and 16HBE cell line. (i) The overexpression efficiency of CORO1C ectopic expression plasmid was analyzed by western blot assay. (j and k) The protein level of CORO1C was determined in NSCLC cells transfected with the following four groups by western blot assay: miR‐NC, miR‐512‐3p, miR‐512‐3p + pcDNA3.1 and miR‐512‐3p + pcDNA3.1‐CORO1C. * p < 0.05

Article Snippet: We collected NSCLC tissue samples and adjacent normal lung tissue samples from 39 NSCLC patients at The Affiliated Nanhua Hospital.

Techniques: Binding Assay, Sequencing, Luciferase, Reporter Assay, Quantitative RT-PCR, Western Blot, Expressing, Over Expression, Plasmid Preparation, Transfection

Journal: bioRxiv

Article Title: Direct measurement of RNA Polymerase II hypertranscription in cancer FFPE samples

doi: 10.1101/2024.02.28.582647

Figure Lengend Snippet: Pathology classification, age and sex were provided by the vendor (BioChain). Each image spans the width of a standard charged microscope slide, where the tissue is visible under the paraffin skin. On-slide RNAPII-Ser5p FFPE-CUTAC was applied to slides in parallel, using a total of four slides each for 100 separate samples in all to produce the data analyzed in this study.

Article Snippet: The following pairs of human tumor and adjacent normal 5 μm tissue sections from single FFPE blocks were purchased from Biochain, Inc: Breast Normal/Tumor cat. no. T8235086PP/PT; Colon Normal/Tumor cat. no. T8235090PP/PT; Kidney Normal/Tumor cat. no. T8235142PP/PT; Liver Normal/Tumor cat. no. T8235149PP/PT; Lung Normal/Tumor cat. no. T8235152PP/PT; Rectum Normal/Tumor cat. no. T8235206PP/PT; Stomach Normal/Tumor cat. no. T8235248PP/PT.

Techniques: Microscopy